FIG. 5.

Homing of Gag-specific CD8⁺ T cells to the colon in Salmonella strain (Sal)-primed macaques. Freshly isolated lymphocytes from endoscope-guided pinch biopsies of the colon were stained with antibodies to CD3 and CD8 in addition to Mamu-A*01 Gag_181-189 tetramers and analyzed by flow cytometry. After gating on the CD3⁺ CD8⁺ T-cell population, the percentage of CD8⁺ T cells specific for Mamu-A*01/Gag_181-189 was determined, as indicated in the upper right quadrants of each plot. More than 100,000 events and at least 6,000 CD3⁺ CD8⁺ T cells were collected for each sample.