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. 2003 Feb;77(4):2559–2567. doi: 10.1128/JVI.77.4.2559-2567.2003

FIG. 6.

FIG. 6.

Comparison of Ad infections in CAR-deficient CHO cells complemented with wt CAR or CAR-LDLR. (A) CAR-deficient cells complemented with wt CAR or CAR-LDLR result in identical Ad infections. Transfected cells were infected with Ad-GFP at an MOI of 5, immunostained for CAR or CAR-LDLR, and analyzed by FACS. Data shown are the numbers of events testing positive in both of the green and red channels out of 10,000 events (n = 3). (B) A representative experiment showing that CAR-deficient cells complemented with wt CAR-eGFP or CAR-LDLR-eGFP result in similar Ad infections. Cells were infected with Ad-RFP at various MOI and analyzed by FACS. The regions shown in green represent cells expressing CAR-GFP or CAR-LDLR-GFP and not infected with Ad-RFP. The regions in red show cells expressing CAR-GFP or CAR-LDLR-GFP and infected with Ad-RFP. The regions in black represent untransfected, uninfected cells. (C) Graphical depiction of data averaged from six experiments as represented by panel B. The y axis represents the percentage of cells testing positive in both of the green and red channels out of total transfected (green) cells.