FIG. 4.

Effects of overexpression of HNF4α (A) and COUP-TF1 (B) on synthesis of pre-C and pregenomic RNAs from the wild-type and CH variant genomes. The autoradiograms show the results of primer extension assays used to quantify the pre-C and pregenomic (preg) RNAs synthesized in Huh7 cells cotransfected with 1 μg of pCDMHNF4 (A) or 0.5 μg of pRSVCOUP-TF1 (B). Quantitations were performed as described in the legends to Fig. 1 and 2. Numbers at the bottom are means ± standard errors of the data relative to the wild type (WT) obtained from three experiments similar to the one for which results are shown.