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. 2003 Feb;77(4):2489–2499. doi: 10.1128/JVI.77.4.2489-2499.2003

FIG. 5.

FIG. 5.

Effects of co-overexpression of HNF4α and COUP-TF1 on synthesis of pre-C and pregenomic (preg) RNAs and viral DNA from wild-type (WT) HBV and the CH variant. Huh7 cells were cotransfected with 3 μg of pWT or pCH, the indicated amounts of HNF4α and COUP-TF1 expression plasmids, and their respective parental vectors at a total concentration of 10 μg of DNA per 60-mm-diameter dish. (A) Autoradiogram showing the results of primer extension assays used to quantify the pre-C and pregenomic RNAs. (B) Autoradiogram showing the results of Southern blot assays used to quantify cytoplasmic viral DNA. RC, DL, and SS are as defined in the legend for Fig. 3. Quantitations were performed as described in the legend to Fig. 1. Numbers at the bottom are means ± standard errors of the data relative to the wild type obtained from three experiments similar to the one for which results are shown.