FIG. 5.

LY294002 inhibits HIV-1 infection of CD4⁺ T cells post-viral entry. Activated CD4⁺ T cells were treated either for 1 h before infection or immediately after virus entry with DMSO, 20 μM LY294002, 100 nM TAK779, 1 μM AMD3100, or 1 mM zidovudine. Twenty-four hours postinfection, cells were harvested, and percent infection was determined as described for Fig. 3. Results are averages of two or more independent experiments for infection with HIV-1 luciferase viruses pseudotyped with JRFL (A), HXB2 (B), or vesicular stomatitis virus (C). For vesicular stomatitis virus (C), LY294002 was also added 24 h postinfection, and cells were harvested after an additional 24 h of incubation.