FIG. 3.

Translational activities of RNA2 derivatives. The accumulation of 2a protein in yeast harboring each of the RNA2 derivatives shown in Fig. 1B was compared by Western blotting of total protein extracts. At the same time, RNA accumulation of these RNA2 derivatives was compared by Northern blotting of total RNA from the same yeast cultures. The relative translational activity of each RNA was calculated as the ratio between the levels of 2a protein and 2a mRNA. The averages from three or more independent experiments are shown.