FIG. 5.

Expressing 2a in trans fails to rescue 1a responsiveness of frameshift RNAs. (A) Schematic diagram of G2aA-GFP, which differs from Ga2A by the addition of the GFP ORF between the 2a ORF and the polyadenylation signal. (B) Membrane association levels of G2aA-fs1 or G2aA-fs2 mRNAs coexpressed with G2aA-GFP mRNA in the absence (−1a) or presence (+1a) of 1a. Cell fractionation was used to assay RNA distribution in the pellet (P) and supernatant (S) fractions as described in the legend to Fig. 1, and representative Northern blots are shown.