FIG. 3.

Quantitation of colocalization of microtubules and OGPy. BMK cells were mock treated (▴) or pretreated with Colcemid (•) and then infected with OGPy in the presence or absence of Colcemid. Samples were fixed and processed for immunodetection of tubulin. After deconvolution, each individual 0.2-μm-thick Z section was examined for total virus particles and particles colocalized with tubulin. The averages of three randomly selected fields from three experiments were taken for each time point.