FIG. 4.

Phylogenetic analysis generated by using the TreePuzzle maximum likelihood method for the translated amino acid sequences of (A) the long polymerase sequence (138 amino acids) and (B) 113 amino acids from the 5′ end of the ORF3 gene (starting from the Bo/Jena/80/DE ORF3 initiation codon) of the UK BoCVs, the German Bo/NLV/Jena/80/DE, and representatives of the human NLV genogroups I and II, with the Sapporo-like virus Hu/Manchester/93/UK used as the outgroup. The numbers at the nodes (quartet puzzling values) indicate the frequencies of occurrence for 1,000 replicate trees. The asterisk indicates the sample identified with the GLPSG1/YGDD1 primer pair.