FIG. 4.

The effects on the pre-RC in infected cells do not require expression of the IE1-72 gene product or late proteins. (A) The cells were G₀ synchronized and either mock infected (M) or infected with HCMV (V) in the absence or presence of 0.36 mM PAA (V+PAA). The cells were harvested after 24 h p.i. and subjected to fractionation, and the soluble (F1) and chromatin-matrix (F4) fractions from an equal number of cells (3 × 10⁵) were analyzed by SDS-PAGE and Western blotting. The Western blot has been overexposed so that the F4 lanes can be visualized. Thus, the exposure for the F1 lanes is out of the linear range. (B) The cells were G₀ synchronized by contact inhibition and mock infected (M), infected with CR208 (dIE1), or infected with Towne strain (V). The cell lysates were prepared at 24 h p.i. and analyzed as in panel A.