FIG. 9.

Binding of MC54L to cells. Empty wells or wells containing confluent CHO, PgsA-745, or BS-C-1 cells in a 48-well plate were preincubated at 4°C with medium containing 10% fetal calf serum for 1 h and containing 1% BSA for another hour and then incubated with recombinant MC54L proteins in medium containing 10% fetal calf serum for 2 h at 4°C. After removal of the medium, the cell monolayers or empty wells were washed extensively and resuspended in SDS-gel loading buffer. The proteins that associated with cells were detected in a Western blot with a MAb against the polyhistidine tag.