FIG. 2.

Immunoblotting of HHV-6A-infected HSB-2 cells. HSB-2 cells were infected with HHV-6A strain U1102 at a multiplicity of infection of 0.1 or mock-infected, and the cells were lysed by RIPA buffer at 72 h postinfection. Lysates were digested with endo H (lanes H) and PNGase F (lanes F), resolved by SDS-PAGE (10% polyacrylamide) under reducing conditions, and electrotransferred onto a PVDF membrane, and the blots were reacted with the anti-gQ MAb, AU100-119. Numbers beside the panels show molecular masses in kilodaltons.