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gB is presented with the kinetics of an early-gene product. PMEFs were infected with HSV-1 KOS or either of the recombinant viruses WSN/NA/gB or WSN/NA/OVA and incubated with gBT-specific, gB-specific (gB498-505), NP-specific (NP366-374) or RR-specific (RR822-829) T cells prior to addition of BFA at increasing intervals. IFN-γ production was determined by flow cytometric analysis of gated CD8+ T cells. NA, no virus. Results of one representative experiment of four are shown.
