FIG. 4.

De novo synthesis of viral proteins is required for in vitro antigen presentation. These experiments were set up as described in the legend to Fig. 1; however, PMEFs were infected with either HSV-1 or UV-inactivated HSV-1 (A) or the recombinant HSV-1 strains KΔ318 or KΔ5C (B). BFA was added to the cultures at various intervals following infection, before flow cytometric analysis of CD8⁺ T cells for IFN-γ production. NA, no virus. Results reflect three independent experiments.