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. 2003 Feb;77(4):2436–2444. doi: 10.1128/JVI.77.4.2436-2444.2003

FIG. 1.

FIG. 1.

Analysis of *AN/L3 purified from infected Sf9 cells by SDS-10% PAGE, followed by staining with Coomassie brilliant blue (A) or by Western blotting with polyclonal antibodies against AN (B) or LEF-3 (C). (A) Lanes 1 to 4, purified *AN/L3: lane 1, 1.25 μg; lane 2, 0.5 μg; lane 3, 0.25 μg; lane 4, 0.13 μg. Lane 5, LEF-3 (0.1 μg); lane M, 10-kDa protein ladder. (B and C) Lanes 1 to 3, purified *AN/L3: lane 1, 130 ng; lane 2, 50 ng; lane 3, 20 ng. Lane 4, LEF-3 (25 ng). Panel C represents the same blot as the panel B after stripping and reprobing with antibodies against LEF-3. The molecular masses of protein markers (in kilodaltons) are shown on the sides of the panels.