FIG. 3.

Sedimentation analysis of *AN/L3. Purified *AN/L3 (100 μg in 90 μl) was layered over 4.8 ml of a 15 to 30% glycerol gradient in buffer containing 0.4 M KCl, 10 mM Tris-HCl (pH 7.5), 1 mM DTT, and 1 mM EDTA and was centrifuged in an SW 50.1 rotor at 46,000 rpm at 4°C for 19 h. The gradient was fractionated from the bottom into 15 fractions, and 5-μl portions from fractions 1 to 14 were analyzed by SDS-10% PAGE followed by Coomassie blue staining. The positions of the standards centrifuged in separate tubes are shown by arrows: catalase (220 kDa, 11.3S), aldolase (158 kDa, 7.7S), BSA (66 kDa, 4.3S), and ovalbumin (45 kDa, 3.5S). The peak fraction of *AN/L3 is indicated by the asterisk.