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. 2003 Feb;23(4):1181–1195. doi: 10.1128/MCB.23.4.1181-1195.2003

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Copyright © 2003, American Society for Microbiology

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FIG. 5. — Comparison of the distributions of BAN, PH, and TRL/GAF proteins on larval salivary polytene chromosomes as detected by double immunostaining. Simultaneous immunostaining was performed for the following combinations: PH-FLAG (green) and BAN (red) (A and B) and BAN-FLAG (green) and TRL/GAF (red) (C and D). In each panel, a merge image is shown in the middle. (B) A representative chromosomal region displaying four adjacent BAN binding sites is enlarged. Among these BAN sites, two are shared with PH (arrowheads) and two are specific for BAN (arrows). In the same region, one PH binding site is not shared with BAN (asterisk). (D) Enlargement of a chromosomal region from panel C shows the colocalization of BAN and TRL/GAF binding sites.