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. 2003 Feb;23(4):1260–1268. doi: 10.1128/MCB.23.4.1260-1268.2003

FIG. 2.

FIG. 2.

Absence of Rap250 mRNA and protein in Rap250−/− embryonic fibroblasts. (A) RT-PCR analysis of total RNA from wild-type (+/+) and Rap250−/− (−/−) mouse embryonic fibroblasts. Expression of Rap250 is detected in wild-type but not null cells, and actin mRNA is detected in both cell types. M, molecular marker (1-kb ladder); 0, negative control without DNA. (B) Western blot analysis of nuclear extracts from wild-type (WT) and null (KO) mouse embryonic fibroblasts using RAP250 polyclonal antibodies. RAP250 protein, indicated by an arrow, is detected in wild-type but not null fibroblasts.