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. 2003 Feb;23(4):1260–1268. doi: 10.1128/MCB.23.4.1260-1268.2003

FIG. 6.

FIG. 6.

Vascular defects in Rap250−/− placentas. The collapse of vessels bordering the labyrinth and the spongiotrophoblast region of Rap250−/− placentas at E13.5 is shown. Immunohistochemical analysis was performed for Rap250−/− (A and C) and Rap250+/− (E and G) extra-embryonic tissues. Panels A, C, E, and G show staining with antibodies against von Willebrand's factor. Note the release of von Willebrand's factor in the collapsed vessel of the labyrinth and the spongiotrophoblast region of the Rap250−/− placenta. In contrast, the placental vessels in the labyrinth region were intact and positive staining was only detected in the endothelial cells. Similar vessels of Rap250+/− placentas were unaffected. (B, D, F, and H) TUNEL staining to detect DNA fragmentation. An area juxtaposed to the collapsed vessels shown in panels B and D stained positive with TUNEL staining (marked with an asterisk). Bars: A, B, E, and F, 125 μm; C, D, G, and H, 50 μm.