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. 2003 Feb;23(4):1151–1162. doi: 10.1128/MCB.23.4.1151-1162.2003

FIG. 1.

FIG. 1.

Immunoblot analysis of HR6A and HR6B expression in germ cells from wild-type and HR6B knockout mouse testis. Highly purified spermatocytes and spermatids were isolated from wild-type (wt), HR6A knockout (aY), and HR6B knockout (bb) mouse testes. (A) Total protein extracts from wild-type cells were run on two-dimensional gels and subjected to isoelectric focusing in the first dimension and SDS-PAGE in the second dimension. HR6A and HR6B were detected with anti-HR6A/B. The positions of the two proteins are indicated; the pH increases from left to right in the gel. (B) Total protein extracts were run on SDS-PAGE gels and then analyzed by using anti-HR6A/B. The position of the 15K molecular weight marker is indicated. The lower panel in B shows part of the Ponceau red-stained blot, with several histone bands, as a control for equal protein loading of the gel. (C) Abundant presence of HR6A and/or HR6B proteins in highly purified spermatocytes (spc), round spermatids (rspt), and elongating spermatids (espt) isolated from wild-type mice.