FIG. 6.

Association of acetylated histones at the histone H4/n locus during HL-60 differentiation. ChIP assays were performed with proliferating HL-60 cells (0) or cells differentiated by treatment with PMA for 1 or 2 days. The antibodies used recognize acetylated histone H4 (α ac. H4) or acetylated histone H3 (α ac. H3). Normal rabbit serum (NRS) was used as a negative control. Precipitated DNA was amplified by PCR using primers detecting specific regions of the H4/n gene, as indicated. A representative set of PCR data from one of two experiments is shown.