FIG. 1.
Targeted disruption of the TAB2 gene. (A) Schematic representation of the targeting strategy. A targeting construct was designed to flank one large exon of the TAB2 gene and the Neor gene with loxP sites (triangles). Cre-mediated removal was performed to generate a flox allele or Δ allele of TAB2. Abbreviations: B, BamHI; E, EcoRI; X, XbaI. Screening primers for PCR are shown as A, B, and C. (B) Southern blot analysis of EcoRI-digested genomic DNA from E11.5-derived MEFs. The probe used is indicated in panel A. (C) Immunoblot analysis of whole-cell extracts from E11.5-derived MEFs using an Ab that recognizes the N terminus of TAB2.