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. 2003 Feb;23(4):1135–1150. doi: 10.1128/MCB.23.4.1135-1150.2003

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FIG. 5. — Effect of Kap104 truncation on pheromone-induced cell cycle arrest. (A) Strains DSY278 and A11 were transformed with YCplac33 (18) (top row) or YCplac33/Gpa1^E364K (36) (bottom row). Strain ZW432 (15Dau kap104Δ::URA3/kap104^Δ827-918) was transformed with YCplac22 (18) (top row) or YCplac22/Gpa1^E364K (62) (bottom row). Transformants were cultured and tested in glucose medium. (B) Immunoblot probed with Gpa1 antibody. Protein was extracted from strains DSY278 (left) and A11 (right), both transformed with YCplac33/Gpa1^E364K. (C) Immunoblot probed with Kap104 antibody. Protein was extracted from strain DSY278 (left) and strain A11 (right).