TABLE 1.
S. cerevisiae strains used in this study
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| 15Dau | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ | 49 |
| DSY245 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ YIplac204/GAL1EG28-GPA1E364K | Lab stock |
| DSY253 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ YIplac128/GAL1EG28-GPA1 (2X) | Lab stock |
| DSY278 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ YIplac204/GAL1EG28-GPA1E364K FUS1-LEU2::HIS2 | 36 |
| A11 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ kap104Δ827-918 YIplac204/GAL1EG28-GPA1E364K FUS1-LEU2::HIS2 | This study |
| ZWY432 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ kap104Δ::URA3 YCplac111/kap104Δ827-918 | This study |
| IHY169 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ msg5Δ::LEU2 YIplac204/GAL1EG28-GPA1E364K FUS1-LEU2::HIS2 | This study |
| HKY137 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ msg5Δ::LEU2 | This study |
| HKY114 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ ptp3Δ::KanMx | This study |
| HKY161 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ msg5Δ::LEU2 ptp3Δ::KanMx | This study |
| IHY165 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ fus3Δ::LEU2 kss1Δ::KanMx | This study |
| IHY205 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ fus3Δ::LEU2 kss1Δ::KanMx YCplac33/FUS3K42R-GFP | This study |
| IHY217 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ fus3Δ::LEU2 kss1Δ::KanMx YIplac204/GAL1EG28-GPA1E364K YCplac33/FUS3K42R-GFP | This study |
| AEY144 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ kap104Δ::HISG YCplac111/kap104Δ827-918 | This study |
| AOY135 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ kap104Δ::HISG YIplac204/GAL1EG28-GPA1E364K YCplac111/kap104Δ827-918 | This study |
| TBY137 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ msg5Δ::LEU2 ptp3Δ::KanMx YIplac204/GAL1EG28-GPA1E364K | This study |
| TBY141 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ ptp3Δ::KanMx YIplac204/GAL1EG28-GPA1E364K | This study |
| TBY147 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ msg5Δ::LEU2 YIplac204/GAL1EG28-GPA1E364K | This study |
| TBY155 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ YIplac204/GAL1EG28-GPA1K21E R22E E364K | This study |
| TBY163 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ msg5Δ::LEU2 ptp3Δ::KanMx YIplac128/GAL1EG28-GPA1 (2×) | This study |
| MMY107 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ gpa1Δ::URA3 YIplac128/GAL1EG43-GPA1 YCplac22/GPA1K21E R22E | 44 |
| DSY419 | MATabar1Δ ade1 his2 leu2-3,112 trp1 ura3Δ gpa1ts Fus1-lacZ::URA3 | Lab stock |