TABLE 2.
Plasmids used in this study
| Plasmid | Marker | Source |
|---|---|---|
| pEL4-1 | URA3 CEN KAP104 | This study |
| kap104Δ827-918/YCplac111 | LEU2 CEN kap104Δ827-918 | This study |
| KAP104/YIplac211 | URA3 integrative KAP104 | This study |
| kap104Δ::URA3/pUC19 | URA3 integrative kap104Δ | This study |
| kap104Δ::hisG-URA3-hisG/pUC19 | URA3 integrative kap104Δ | This study |
| pYES/KAP104 | URA3 2μ GAL1-KAP104 | This study |
| pIH4.1 | URA3 CEN FUS3-GFP | This study |
| pIH4.1AA | URA3 CEN FUS3T180A Y182A-GFP | This study |
| pIH4.1KM1-1 | URA3 CEN FUS3K42R-GFP | This study |
| YCplac22/FUS3-GFP (AOB117) | TRP1 CEN FUS3-GFP | This study |
| pRS314Nab2-GFP | URA3 CEN NAB2-GFP | 14 |
| pRS316CG/MSG5-GFP (HKB101) | URA3 CEN MSG5-GFP | This study |
| YCplac111/GAL1-MSG5 (HKB100) | LEU2 CEN GAL1-MSG5 | This study |
| YCplac22/GAL1-MSG5 (AEB101) | TRP1 CEN GAL1-MSG5 | This study |
| YCplac111/Gpa1E364K (DSB117) | LEU2 CEN GPA1E364K | 61 |
| YCplac33/Gpa1E364K (HSB105) | URA3 CEN GPA1E364K | 36 |
| YCplac22/GPA1K21E R22E | TRP1 CEN GPA1K21E R22E | 44 |
| YIplac204/GAL1EG28-GPA1E364K | TRP1 GPA1E364K | 62 |
| YIplac204/GAL1EG28-GPA1K21E R22E E364K (TBB105) | TRP1 GPA1K21E R22E E364K | This study |
| YIplac128/GAL1EG28-GPA1 | LEU2 GPA1 | 62 |
| YCplac111/GAL1-PTP3 (TBB104) | LEU2 CEN GAL1-PTP3 | This study |