FIG. 4.

Mutation of the CaM binding sequences does not affect DNA binding or IκB inhibition properties of RelA or c-Rel. RelA (A) or c-Rel (B) and their respective CaM binding-deficient derivatives were in vitro translated by using a rabbit reticulocyte lysate system and analyzed for DNA binding activity by EMSA with a κB sequence as probe. Where indicated (+), proteins were preincubated with E. coli-produced and purified IκBα before addition of DNA. In the first lane of each gel, no protein was added to the binding reaction. lysate, binding reactions with rabbit reticulocyte lysate without in vitro-translated protein.