TABLE 1.
DNA substrate | Incoming nucleotide | kcat (min−1) | Km (μM) | kcat/Km (min−1 μM−1) | Relative incorporation efficiency |
---|---|---|---|---|---|
Insertion opposite G | |||||
5′---CAT | dGTP | 0.06 ± 0.008 | 420 ± 70 | 0.00014 | 1.1 × 10−4 |
---GTAGAAT--- | dATP | 0.02 ± 0.01 | 370 ± 100 | 0.000054 | 4.3 × 10−5 |
dTTP | 0.03 ± 0.006 | 450 ± 80 | 0.000067 | 5.4 × 10−5 | |
dCTP | 0.44 ± 0.02 | 0.36 ± 0.03 | 1.23 | 1 | |
Insertion opposite 8oxoG | |||||
5′---CAT | dGTP | NDa | ND | ||
---GTAGAAT | dATP | 0.11 ± 0.02 | 6.3 ± 0.4 | 0.017 | 1.4 × 10−2 |
8oxo | dTTP | ND | ND | ||
dCTP | 0.09 ± 0.03 | 7.5 ± 1 | 0.012 | 9.7 × 10−3 | |
Insertion opposite m6G | |||||
5′---CAT | dGTP | 0.02 ± 0.007 | 430 ± 80 | 0.000047 | 3.8 × 10−5 |
---GTAGAAT | dATP | ND | ND | ||
m6 | dTTP | 0.08 ± 0.008 | 46 ± 11 | 0.0017 | 1.4 × 10−3 |
dCTP | 0.02 ± 0.004 | 370 ± 90 | 0.000054 | 4.4 × 10−5 |
ND, not detected.