Skip to main content
. 2003 Feb;23(4):1453–1459. doi: 10.1128/MCB.23.4.1453-1459.2003

TABLE 1.

Steady-state kinetic parameters of nucleotide insertion reactions opposite 8-oxoG and m6G template residues by yeast Polζ

DNA substrate Incoming nucleotide kcat (min−1) Km (μM) kcat/Km (min−1 μM−1) Relative incorporation efficiency
Insertion opposite G
    5′---CAT dGTP 0.06 ± 0.008 420 ± 70 0.00014 1.1 × 10−4
---GTAGAAT--- dATP 0.02 ± 0.01 370 ± 100 0.000054 4.3 × 10−5
dTTP 0.03 ± 0.006 450 ± 80 0.000067 5.4 × 10−5
dCTP 0.44 ± 0.02 0.36 ± 0.03 1.23 1
Insertion opposite 8oxoG
    5′---CAT dGTP NDa ND
---GTAGAAT dATP 0.11 ± 0.02 6.3 ± 0.4 0.017 1.4 × 10−2
8oxo dTTP ND ND
dCTP 0.09 ± 0.03 7.5 ± 1 0.012 9.7 × 10−3
Insertion opposite m6G
    5′---CAT dGTP 0.02 ± 0.007 430 ± 80 0.000047 3.8 × 10−5
---GTAGAAT dATP ND ND
m6 dTTP 0.08 ± 0.008 46 ± 11 0.0017 1.4 × 10−3
dCTP 0.02 ± 0.004 370 ± 90 0.000054 4.4 × 10−5
a

ND, not detected.