TABLE 2.

Steady-state kinetic parameters of extension reactions opposite from 8-oxoG and m6G template residues by yeast Polζ

DNA substrate	Incoming nucleotide	kcat (min−1)	Km (μM)	kcat/Km (min−1 μM−1)	Relative extension efficiency
Extension from G, A, T and C opposite G
5′---CATG
---GTAGAAT---	dTTP	0.22 ± 0.01	7.2 ± 0.8	0.03	5 × 10−2
5′---CATA
---GTAGAAT---	dTTP	0.2 ± 0.02	2.4 ± 0.3	0.083	1.4 × 10−1
5′---CATT
---GTAGAAT---	dTTP	0.16 ± 0.02	7.8 ± 0.9	0.02	3.3 × 10−2
5′---CATC
---GTAGAAT---	dTTP	0.23 ± 0.02	0.38 ± 0.05	0.6	1
Extension from G, A, T and C opposite 8oxoG
5′---CATG
---GTAGAAT----
8oxo	dTTP	0.19 ± 0.02	7.6 ± 0.9	0.025	4.1 × 10−2
5′---CATA
---GTAGAAT----
8oxo	dTTP	0.21 ± 0.01	0.48 ± 0.05	0.44	7.3 × 10−1
5′---CATT
---GTAGAAT----
8oxo	dTTP	0.08 ± 0.03	34 ± 7	0.0024	4 × 10−3
5′---CATC
---GTAGAAT----
8oxo	dTTP	0.15 ± 0.02	1.5 ± 0.2	0.1	1.7 × 10−1
Extension from G, A, T and C opposite m6G
5′---CATG
---GTAGAAT----
m6	dTTP	0.18 ± 0.03	2.0 ± 0.1	0.09	1.5 × 10−1
5′---CATA
---GTAGAAT----
m6	dTTP	0.07 ± 0.01	6.9 ± 0.5	0.01	1.7 × 10−2
5′---CATT
---GTAGAAT----
m6	dTTP	0.08 ± 0.009	4.3 ± 0.6	0.019	3.1 × 10−2
5′---CATC
---GTAGAAT----
m6	dTTP	0.16 ± 0.03	1.4 ± 0.1	0.11	1.9 × 10−1