Skip to main content
PMC home page
Gut logoLink to Gut
. 1977 Oct;18(10):779–785. doi: 10.1136/gut.18.10.779

Immunohistochemistry of carcinoembryonic antigen: characterisation of cross-reactions with other glycoproteins.

P Isaacson, M A Judd
PMCID: PMC1411693  PMID: 73495

Abstract

In the course of demonstrating carcinoembryonic antigen (CEA) in normal human small intestine cross-reactivity of specific antiserum against red blood cells, vascular endothelium, and Paneth cell granules was noted. Pretreatment of sections with periodic acid eliminated these cross-reactions without affecting the staining of CEA, indicating that the antigenic determinants shared between CEA and other glycoproteins are in the carbohydrate portion of the molecules. These findings emphasise the caution with which immunohistochemical results should be regarded even when they are apparently well controlled.

Full text

PDF
785

Images in this article

783Fig. 6
on p.783
783Fig. 7
on p.783
783Fig. 8
on p.783
782Fig. 3
on p.782
782Fig. 4
on p.782
782Fig. 5
on p.782
781Fig. 1
on p.781
781Fig. 2
on p.781

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burtin P., Quan P. C., Sabine M. C. Nonspecific cross reacting antigen as a marker for human polymorphs, macrophages and monocytes. Nature. 1975 Jun 26;255(5511):714–716. doi: 10.1038/255714a0. [DOI] [PubMed] [Google Scholar]
  2. Cooper A. G., Brown M. C., Kirch M. E., Rule A. H. Relationship of carcinoembryonic antigen to blood substances A and i: evidence that the antigenic sites are on different molecules. J Immunol. 1974 Oct;113(4):1246–1251. [PubMed] [Google Scholar]
  3. Darcy D. A., Turberville C., James R. Immunological study of carcinoembryonic antigen (CEA) and a related glycoprotein. Br J Cancer. 1973 Aug;28(2):147–160. doi: 10.1038/bjc.1973.132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldenberg D. M., Sharkey R. M., Primus F. J. Carcinoembryonic antigen in histopathology: immunoperoxidase staining of conventional tissue sections. J Natl Cancer Inst. 1976 Jul;57(1):11–22. doi: 10.1093/jnci/57.1.11. [DOI] [PubMed] [Google Scholar]
  5. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  6. Heyderman E., Neville A. M. Immunoperoxidase localisation of pregnancy protein. Lancet. 1976 Oct 2;2(7988):744–744. doi: 10.1016/s0140-6736(76)90043-x. [DOI] [PubMed] [Google Scholar]
  7. Holburn A. M., Mach J. P., MacDonald D., Newlands M. Studies of the association of the A, B and Lewis Blood group antigens with carcinoembryonic antigen (CEA). Immunology. 1974 Apr;26(4):831–843. [PMC free article] [PubMed] [Google Scholar]
  8. Huitric E., Laumonier R., Burtin P., Von Kleist S., Chavanel G. An optical and ultrastructural study of the localization of carcinoembryonic antigen (CEA) in normal and cancerous human rectocolonic mucosa. Lab Invest. 1976 Jan;34(1):97–107. [PubMed] [Google Scholar]
  9. Isaacson P., Judd M. A. Carcinoembryonic antigen (CEA) in the normal human small intestine: a light and electron microscopic study. Gut. 1977 Oct;18(10):786–791. doi: 10.1136/gut.18.10.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Isaacson P., Le Vann H. P. The demonstration of carcinoembryonic antigen in colorectal carcinoma and colonic polyps using an immunoperoxidase technique. Cancer. 1976 Sep;38(3):1348–1356. doi: 10.1002/1097-0142(197609)38:3<1348::aid-cncr2820380339>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  11. Isaacson P. Tissue demonstration of carcinoembryonic antigen (CEA) in ulcerative colitis. Gut. 1976 Jul;17(7):561–567. doi: 10.1136/gut.17.7.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Khoo S. K., Warner N. L., Lie J. T., Mackay I. R. Carcinoembryonic antigenic activity of tissue extracts: a quantitative study of malignant and benign neoplasms, cirrhotic liver, normal adult and fetal organs. Int J Cancer. 1973 May;11(3):681–687. doi: 10.1002/ijc.2910110319. [DOI] [PubMed] [Google Scholar]
  13. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  14. Primus F. J., Wang R. H., Sharkey R. M., Goldenberg D. M. Detection of carcinoembryonic antigen in tissue sections by immunoperoxidase. J Immunol Methods. 1975 Sep;8(3):267–275. doi: 10.1016/0022-1759(75)90121-0. [DOI] [PubMed] [Google Scholar]
  15. TAYLOR J. J., FLAA R. C. HISTOCHEMICAL ANALYSIS OF PANETH CELL GRANULES IN RATS. Arch Pathol. 1964 Mar;77:278–285. [PubMed] [Google Scholar]
  16. Turberville C., Darcy D. A., Laurence D. J., Johns E. W., Neville A. M. Studies on carcinoembryonic antigen (CEA) and a related glycoprotein, CCEA-2. Preparation and chemical characterisation. Immunochemistry. 1973 Dec;10(12):841–843. doi: 10.1016/0019-2791(73)90189-4. [DOI] [PubMed] [Google Scholar]
  17. Westwood J. H., Thomas P. Studies on the structure and immunological activity of carcinoembryonic antigen - the role of disulphide bonds. Br J Cancer. 1975 Dec;32(6):708–719. doi: 10.1038/bjc.1975.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. von Kleist S., Chavanel G., Burtin P. Identification of an antigen from normal human tissue that crossreacts with the carcinoembryonic antigen. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2492–2494. doi: 10.1073/pnas.69.9.2492. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES