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. 2003 Feb;2(1):76–83. doi: 10.1128/EC.2.1.76-83.2003

FIG. 4.

FIG. 4.

Dimerization of GPI-minus VSG. Detergent extracts of radiolabeled transgenic bloodstream cells (5-min pulse, 5-min chase) were fractionated by velocity sedimentation. The sedimentation positions of transgenic 117Δgpi (A) and 221Δgpi (B) and endogenous, GPI-anchored VSG (data not shown) were determined by immunoprecipitation. Samples of each fraction were analyzed by SDS-PAGE, and the sedimentation positions of internal molecular mass standards (c = carbonic anhydrase, 31 kDa; b = bovine serum albumin, 68 kDa; γ = bovine gamma globulin, 170 kDa) were determined by Coomassie blue staining. The peak positions of internal markers and endogenous VSG are indicated. The scale refers to the molecular mass in kilodaltons.