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. Author manuscript; available in PMC: 2006 Mar 24.
Published in final edited form as: J Neurochem. 2005 Feb;92(3):585–596. doi: 10.1111/j.1471-4159.2004.02886.x

Fig. 1.

Fig. 1

Overexpression of Nex1 promotes survival of PC12-Nex1 cells upon serum deprivation. (a) Prolonged survival of PC12-Nex1 cells (clones A, B and C) compared with control PC12-bsd and PC12-Nex1-mut1 cells after serum deprivation. Trypan blue exclusion test results are expressed as percentage survival. (b) Overexpression of Nex1 results in increased cell viability, as measured by LDH assay. Values represent the mean ± SEM (n = 9) of three independent experiments.(c) Overexpression of Nex1 prevents DNA fragmentation upon serum deprivation in PC12-Nex1 cells compared with control PC12-bsd cells. Apoptotic DNA was isolated from PC12-bsd and PC12-Nex1 cells at different time points during serum deprivation treatment, according to the manufacturer's recommendations. Equal amounts of DNA were run on a 1.5% agarose gel and stained with ethidium bromide. DNA markers were electrophoresed as a base pair reference. (d) PC12-Nex1 cells (clones A, B, and C) remain metabolically active after a prolonged 15-day serum deprivation treatment. The metabolic activity of PC12-Nex1 cells was measured by a colorimetric WST-1 assay. Data represent mean ± SD of three independent experiments performed in triplicate.