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. Author manuscript; available in PMC: 2006 Mar 24.
Published in final edited form as: J Neurochem. 2005 Feb;92(3):585–596. doi: 10.1111/j.1471-4159.2004.02886.x

Fig. 5.

Fig. 5

Nex1 promotes long-term survival of serum-deprived Pc12-Nex1 cells, independently of the Akt pathway. (a) Serum deprivation of PC12-Nex1 cells does not lead to phosphorylation of Akt at Ser-437. PC12-Nex1 cells were deprived of serum for the times indicated above the panel and equal amounts of total protein were subjected to immunoblot analysis. Amounts of total and phosphorylated Akt were detected by using anti-Akt and anti-phosphorylated Akt (Ser-437) antibodies respectively. The antigen—antibody complexes were detected by chemiluminescence. The membrane was stripped before probing with the anti-Akt antibody. Molecular weight markers are indicated on the left side of each figure. Data shown are representative of at least three independent experiments. (b) The majority of PTEN protein exists in an inactive phosphorylated form in serum-deprived PC12-Nex1 cells. PC12-Nex1 cells were deprived of serum for times indicated above the panel and whole-cell extracts were analyzed by immunoblotting. Amounts of total and phosphorylated PTEN were detected by using anti-PTEN and anti-phosphorylated PTEN (Ser-380) antibodies respectively. The antigen—antibody complexes were detected by chemiluminescence. The membrane was stripped before probing with the anti-phosphorylated PTEN antibody. Data shown are representative of at least three independent experiments.