Table 1.
Primary antibodies used in this study
| Antibody | Type | Source (Catalog no.) | Remarks |
|---|---|---|---|
| Nex1 | Rabbit pAb | Chiaramello laboratory | Uittenbogaard and Chiaramello (2002) |
| βIII tubulin | Mouse mAb | Covance (mms-435P) | |
| GAP-43 | Rabbit pAb | Chemicon (AB 5220) | |
| p21waf/cip | Mouse Ab | Santa-Cruz (sc-6246) | |
| p27KIP1 | Rabbit pAb | StressGen (KAP-CC02) | |
| p16INK4 | Rabbit pAb | SantaCruz (sc-1207) | |
| CDC47 | Mouse mAb | Neomarkers (MS-862) | |
| Bcl-xL | Rabbit pAb | Santa Cruz (sc-7195) | Specific to Bcl-xL only (amino acids 126-188) |
| Bcl-w | Rabbit pAb | Santa Cruz (sc-11422) | Recognizes (amino acids 55-193) |
| XIAP | Mouse mAb | StressGen (AAM-050) | |
| Survivin | Rabbit pAB | Chemicon (AB 3611) | |
| PTEN | Rabbit pAb | Upstate (07-016) | |
| p-PTEN | Rabbit pAb | Cell Signaling (9551) | Recognizes only p-PTEN at ser380 |
| Akt | Rabbit pAb | Cell Signaling (9272) | |
| p-Akt | Mouse mAb | Cell Signaling (4057) | Recognizes only p-Akt at ser473 |
| PARP | Rabbit pAb | Cell Signaling (9545) | Recognizes cleaved PARP (larger fragment only) |
mAb monoclonal antibody; pAb, polyclonal antibody; p-PTEN, phosphorylated form of PTEN; p-Akt, phosphorylated form of Akt.