Table 5.
Effects of Various Inhibitors on Cytoplasmic Motility in Epidermal Cells of Vallisneria
| Experiment 1 | n | After Treatment | +WL | After Wash | +WL |
|---|---|---|---|---|---|
| Control | 5 | 14.1 ± 0.4 | 16.2 ± 0.5 | ||
| Latrunculin B | 5 | 11.9 ± 0.3a | 11.8 ± 0.4 | 14.0 ± 0.5 | 17.2 ± 0.5 |
| Cytochalasin B | 4 | 12.1 ± 0.1a | 12.0 ± 0.3 | 14.0 ± 0.6 | 16.9 ± 1.3 |
| Colchicine | 5 | 14.2 ± 0.4 | 16.5 ± 0.7 | ||
| Experiment 2 | n | After Treatment | +R | ||
| Control | 3 | 13.9 ± 0.9 | 15.2 ± 1.0 | ||
| Vanadate | 5 | 13.8 ± 1.2 | 15.1 ± 1.2 | ||
| La3+ | 5 | 13.8 ± 0.9 | 15.3 ± 1.1 |
Dark-adapted samples were treated in darkness with 0.1 μM latrunculin B for 1 h, 0.1 mM cytochalasin B for 3 h, 1 mM colchicine for 6 h, 0.2 mM vanadate for 1 h, or 0.1 mM La3+ for 1 h. In the treated samples, the index of cytoplasmic motility was determined immediately before and after irradiation with white light (+WL; 2 W/m2, 4 s) or red light (+R; 100 μmol·m−2·s−1, 1 s). After treatment with latrunculin B and cytochalasin B, the reagent was removed by vigorous washing of the treated samples with artificial pond water for 2 or 4 h, respectively, and then the photoinduction of cytoplasmic motility was examined in the washed samples. For each treatment, the response is expressed as the average with the standard error. Control, treatment with normal artificial pond water; n, the number of samples examined.
Significantly different from the initial value of the index (13.9 ± 0.2) determined immediately before the start of treatment with reagents (P < 0.02).