Figure 3.

HR Phenotypes of hlm1 Plants in Response to Diverse Avirulent Pathogens.

(A) to (D) Leaves from wild-type (Ws-4) and hlm1 plants after inoculation with Pseudomonas DC3000 containing the avrRpt2 gene ([A] and [C]) or the avrRps4 gene ([B] and [D]) at 48 h after inoculation (hpi; [A] and [B]) or 6 days after inoculation (dpi; [C] and [D]).

(E) and (F) Leaves from wild-type (Ws-4) and hlm1 plants after inoculation with the avirulent strain of Xanthomonas (Xcc 147) at 48 h (E) and 6 days (F) after inoculation.

Dots on the leaves indicate inoculated leaves. Three days after inoculation with strains 147, DC3000/avrRps4, and DC3000/avrRpt2, 10, 11, and 27% of the inoculated leaves, respectively, responded when inocula of 5 × 10⁷ cfu/mL were used, whereas 100% of leaves responded in the case of wild-type control plants. Six days after inoculation, 80, 80, and 71% of leaves responded to the same strains, respectively, showing either chlorotic symptoms (avrRps4 and Xanthomonas strain 147) or HR-like symptoms (avrRpt2).

(G) Evaluation of cell death in wild-type (open bars) and hlm1 (closed bars) leaves after inoculation with avirulent strain 147 of Xanthomonas. Uptake of Evans blue by leaves was quantified by spectrophotometry. Data are expressed as OD units and result from four independent experiments.