Figure 1.

Expression Patterns of the CIPK3 Gene.

(A) RT-PCR analysis of CIPK3 transcripts during seed germination and in different organs of Arabidopsis plants. Total RNA was isolated from various tissues (root, leaf, stem, flower, and silique) of 4-week-old wild-type plants grown under long-day conditions or from germinating seeds and young seedlings (3, 5, and 7 days after sowing). RT-PCR was performed with either CIPK3-specific primers (top gel) or Actin2-specific primers (bottom gel).

(B) Histochemical GUS analysis of CIPK3 promoter–GUS transgenic plants. Top left, a germinating seed; bottom left, a 2-week-old seedling; top right, a rosette leaf from a 4-week-old plant; bottom right, a flower showing faint blue color in the sepal only.

(C) Accumulation of CIPK3 gene transcripts in response to cold (4°C), NaCl (300 mM), ABA (100 μM), drought, or wounding treatment. Each lane was loaded with 10 μg of total RNA from 3-week-old Arabidopsis plants. The numbers indicate hours after each treatment.