Figure 4.

Expression of Stress-Responsive Genes in Wild-Type (Ws-0) and cipk3 Mutant Plants.

(A) Expression of stress genes under stress conditions and ABA application. RNA gel blot analysis of RD29A, KIN1, and KIN2 in plants after treatment with cold (4°C for 12 h), ABA (100 μM for 5 h), NaCl (300 mM for 3 h), and water control (3 h).

(B) Transcript levels of CIPK3 and RD29A in Ws-0, cipk3 mutant, and a complementation line (cipk3/CIPK3) after cold treatment for 12 h.

rRNA on the membrane was visualized by staining with methylene blue as an equal loading control.