Figure 5.

The eto2 Mutation Does Not Affect the in Vivo Specific Activity of ACS5.

(A) Quantification of myc fusion protein levels from myc-ACS5^WT and myc-ACS5^eto2 transgenic lines. Three-day-old etiolated seedlings were grown in the presence of various concentrations of DEX, and the proteins were extracted and analyzed by immunoblotting using an anti-myc monoclonal antibody. The relative level of myc antigen in each sample was quantified using a densitometer as described in Methods and is plotted as arbitrary units. The inset shows an image of the original immunoblot.

(B) Ethylene production from seedlings grown at various concentrations of DEX. The myc-ACS5^WT and myc-ACS5^eto2 transgenic lines were grown as in (A), and the amount of ethylene produced during the course of 3 days was measured as described in Methods.