Figure 8.

The eto1 Mutation Acts Partially through the ACS5 Isoform.

(A) Seedling phenotypes of the wild type (WT), eto1, and eto1 cin5 double mutants (two different F2 lines). Seedlings were grown for 3 days in the dark on MS medium in air or ethylene as indicated, and representative seedlings were picked and photographed. Note that the double mutant lines have a phenotype intermediate between the wild-type and the eto1 parental lines.

(B) Ethylene production from various lines. Seedlings of the indicated genotypes were grown for 4 days in the dark on MS medium in capped GC vials, and the amount of accumulated ethylene was measured as described in Methods. The lines all are homozygous for the indicated mutations. The ecotype of each line is noted below the graph (Col/Ws indicates that the line is the F3 generation of a cross between Col and Ws parents). The original eto1 allele is in the Col ecotype, and cin5 is in Ws. Two independent F3 lines from a backcross of eto1 to a Ws line also are shown (eto1 Col/Ws).