Figure 1.

Expression of Arabidopsis BiP Genes after Treatment with Tunicamycin and DTT.

Six-day-old Arabidopsis seedlings growing in liquid were treated with 5 μg/mL tunicamycin (Tun) or 10 mM DTT for 2 or 5 h. Control assays were performed at 0, 2, and 5 h. Tunicamycin was added to the 0-h control, and DMSO was added to the 2- and 5-h controls. Total RNA was fractionated on a formaldehyde-agarose gel, transferred onto a nylon membrane, and then hybridized with ³²P-labeled AtBiP2 probe. Ethidium bromide staining under UV light was used to evaluate equal loading (bottom gels).