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The SAM domains of Ste11p and Ste50p are functionally exchangeable. (A) Schematic representation of the SAM domain-swapped chimeras of Ste11p and Ste50p. (B) Yeast cells (ste11Δ ste50Δ ssk2Δ ssk22Δ) were cotransformed with either the wild-type STE11 and STE50 or SAM domain-swapped chimeras of STE11 and STE50, and tested for the ability to grow on hyperosmotic medium. (−ura) Synthetic dextrose uracil drop-out medium; (+sorbitol) selective medium with 1.5 M sorbitol.
