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. 2006 Mar 15;20(6):734–746. doi: 10.1101/gad.1375706

Figure 5.

Figure 5.

Plasma membrane localization of Ste11p leads to simultaneous activation of multiple signaling pathways. (A) Yeast cells of genotypes as indicated transformed with various STE11 constructs or with the control vector were assayed for their ability to grow on hyperosmotic media. (B) The morphology of yeast cells with relevant genotype (indicated at the bottom of the figure) transformed either with myristoylated GST-Ste11p (top) or with GST-Ste11p (bottom). The yeast strains used were W303-1A (wt), YEL206 (ste20Δ), YCW555 (ste50Δ ste11Δ ssk2Δ ssk22Δ), and YCW757 (ste50Δ ste5Δ ste11Δ ssk2Δ ssk22Δ). (C) Ste11p plasma membrane localization is sufficient for activation of the HOG pathway, but still needs Ste5p for mating pathway activation. Yeast cells of the indicated genotype (top) transformed with STE11 constructs as indicated on the right were assayed for their ability to grow on hyperosmotic medium (middle), and to form diploids (right). (−ura) Synthetic dextrose uracil drop-out medium; (−ura/gal) synthetic uracil drop-out with galactose as carbon source; (+sorbitol) with 1.5 M sorbitol; (MM) minimal medium.