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Effect of astCADBE depleted arginine on argCBH expression in wild type, ΔrpoS, Δast ,and ΔrpoS Δast strains. Strains were grown in triplicate in LB rich media. Samples were taken in exponential phase (□, OD600 = 0.3) and stationary phase (■, OD600 = 1.5), and β-glactosidase activity was assayed as described in Methods.
