Table 4.
Primer pairs used for PCR amplification of unique and differential regions in 18 Brucella biovars. Sequences of primer pairs, which where use to PCR amplify several differential genes across the 18 Brucella biovars along with the expected amplicon size and predicted gene function.
| Primer pair number | Forward primer ... Reverse primer | ORF name Amplicon size, bp | Gene function | ||
| B. abortus | B. melitensis | B. suis | |||
| 1 | TGATAGCGCCAGACAACAAC ... TGTGCCAGCTTCGTTGTAAG | BruAb1_1825 596 | BMEI0205 470 | BR1846 722 | Immunoglobulin-binding protein EIBE |
| 2 | AAATGTCAATCTGGGCTTCG ... TATTGAAGAACTGCGCAACG | BRA0378 191 | Hypothetical protein | ||
| 3 | ATTTATGTCCGTGAACTGTCCGTC ... TTGTCCGCAAAAAGTATCAAAACG | BRA0369 123 | Hypothetical protein | ||
| 4 | AACTGCTGGAGATGAATCCG ... GAATGTTTGCACGCATCAAT | BRA0363 149 | DNA-binding protein | ||
| 5 | CTTTACGCCCGTGTATCGAC ... CATGGGGTCCTGTGTTGAG | BMEI1661 321 | Recombinase | ||
| 6 | TGCAGCTCACGGATAATTTG ... ACACCTTGTCCACGCTCAC | BruAb2_0168 783 | Outermembrane transporter | ||
| 7 | AGCTTCTGGAGGAGGTGGAT ... GTTCCGCCTTGTGTTTCTTC | BMEII0827 526 | BRA0439 526 | Glucose-1-phosphate cytidylyltransferase | |
| 8 | TCTACACCACGCTGAAGTCG ... CCGAAAGCCGATAGAGTTTG | BruAb2_1035 393 | BMEII0204 162 | BRA1096 393 | Transcriptional regulator, GNTR family |
| 9 | TTGTTGGAAACGGCTTTGATATC ... GAAAGTACCCACCCTCGGAAAACT | BruAb1_0266 358 | BMEI1681 358 | Hypothetical protein | |
| 10 | TCATGCTGTGCCTCCAATTCC ... TTGCTGAGCAGCAGCAAGAAC | BruAb1_0248 184 | BMEI1699 184 | Hypothetical protein | |
| Control | TCAGGCGCTTATAACCGAAG ... ATCTGCGCATAGGTCTGCTT | BruAb2_0582 261 | BMEII0637 261 | BRA0644 261 | pcaC 4-carboxymuconolactone decarboxylase |