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. 2006 Jan 30;103(6):1988–1993. doi: 10.1073/pnas.0505667103

Fig. 4.

Fig. 4.

Effects of overexpression of the phosphorylated active form of AREB1 on plant growth and expression of the RD29B gene. (A) Three and one independent lines of transgenic plants harboring 35S:AREB1pa and the pBI121 vector (pBI121) were grown on germination media agar plates, respectively. The relative amount of the AREB1 and RD29B mRNAs in the transgenic plants was analyzed by quantitative RT-PCR (amount of the most abundant point was indicated as 100). Total RNA was prepared from 3-week-old seedlings of transgenic plants. Pictures of 3-day-old (B) and 9 day-old (C) seedlings. (D) Total RNA was prepared from 3-week-old seedlings of transgenic plants treated with or without 50 μM ABA. The relative amount of the RD29B mRNA in the transgenic plants was analyzed by quantitative RT-PCR and was indicated after considering the nontreated vector control as “1.”