Table 1.
Biacore data for the selected family clones
| Clone | Ka (1/Ms) | Kd (1/s) | KD (nM) |
|---|---|---|---|
| PBLA8 | 0.9 × 105 | 1.0 × 10−4 | 1.0 (0.2) |
| PBLB11 | 3.0 × 105 | 1.4 × 10−3 | 5.0 (1.6) |
| Epi6 | 2.1 × 105 | 1.6 × 10−3 | 8.5 (4.8) |
| Ancestral | 2.1 × 105 | 2.0 × 10−3 | 9.5 (0) |
| PBLB5 | 0.5 × 105 | 1.5 × 10−3 | 30 (8.5) |
Affinity constants (KD) were calculated as association constant (Ka) over dissociation constant (Kd), and the mean for each was calculated; SDs are indicated in parentheses. Data are the means of two to three experimental repeats with duplicate runs per experiment.