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Biacore data for the ancestral mutants A30V and S61R
| Clone | Ka (1/Ms) | Kd (1/s) | KD (nM) |
|---|---|---|---|
| Ancestral | 2.1 × 105 | 2.0 × 10−3 | 9.5 (0) |
| A30V | 1.4 × 105 | 1.6 × 10−3 | 10 (1.3) |
| S61R | 3.5 × 105 | 1.2 × 10−3 | 3.3 (0.07) |
Affinity constant (KD) is calculated as for data in Table 1. SDs are indicated in parentheses.
