Fig. 2.

Development of CD4⁺ 2C T cells in 2C⁺RAG^−/− mice requires MHC class II I-A^b. PBMC from 2C⁺RAG^−/−I-A^b+/+, 2C⁺RAG^−/−I-A^b+/−, and 2C⁺RAG^−/−I-A^b−/− mice were analyzed for 2C TCR, CD4, and CD8 expression. (A) A representative histogram showing 2C TCR expression by all PBMC and dot plots showing CD4 versus CD8 expression gating on 2C⁺ T cells for representative 2C⁺RAG^−/−I-A^b+/− and 2C⁺RAG^−/−I-A^b−/− mice. The numbers indicate percentages of cells in each quadrant. (B and C) Percentages of CD4⁺ 2C⁺ T cells and CD8⁺ 2C⁺ T cells in PBMC are shown for 2C⁺RAG^−/−I-A^b+/+, 2C⁺RAG^−/−I-A^b+/−, and 2C⁺RAG^−/−I-A^b−/− mice. One symbol represents one mouse. The horizontal line indicates the average within each genotype.