Fig. 3.

Development of CD4⁺ T cells in 2C⁺RAG^−/− mice (A) and OT-1⁺RAG^−/− mice (B). (A) PBMC from 2C⁺RAG^−/−TAP^+/+ and 2C⁺RAG^−/−TAP^−/− mice were analyzed for 2C TCR, CD4, and CD8 expression. CD4 versus CD8 expression profiles are shown for 2C⁺ T cells. (B) PBMC of OT-1⁺RAG^−/−TAP^+/+, OT-1⁺RAG⁺TAP^−/−, and OT-1⁺RAG^−/−TAP^−/− mice were stained with anti-Vα2, anti-CD4, and anti-CD8. CD4 versus CD8 expression profiles are shown for Vα2⁺ T cells. The numbers indicate the percentages of T cells in the specific quadrants. Data from representative mice are shown.