Fig. 4.

Surface phenotype of CD4⁺ 2C T cells in 2C⁺RAG⁺TAP^−/− mice. (A) Lymph node cells of 2C⁺RAG⁺TAP^−/− mice were analyzed for 2C TCR, CD4, and CD8. (Left) 2C TCR versus CD4 staining profile of all live cells. (Center) CD4 versus CD8 expression profile gating on 2C⁺ T cells. (Right) Comparison of 2C TCR levels among 2C⁺CD4⁺, 2C⁺CD8⁺, and 2C⁺CD4⁻CD8⁻ T cells. Data from one representative mouse are shown. (B) Lymph node cells of 2C⁺RAG⁺TAP^−/− mice were analyzed for 2C TCR, CD4, plus CD44, CD25, or CD45RB. The expression of CD44, CD25, and CD45RB is shown for 2C⁺CD4⁺, 2C⁺CD4⁻, and 2C⁻CD4⁺ T cells.